《植物生理学报》 2021, 57(3): 723-726
萱草花粉电击转化参数的选择
通信作者:倪迪安;E-mail: dani@sit.edu.cn
摘 要:
以萱草品种‘斯特拉’成熟花粉为材料, 比较了不同电场强度和电击次数对花粉转化率的影响。结果显示: 萱草花粉电击转化的最佳参数为1 kV·cm–1、电击3次。
关键词:萱草; 花粉; 电击转化
收稿:2020-11-28 修定:2021-01-27
资助:上海市科技兴农项目[沪农科推字(2019)第1-8号]
Corresponding author: NI Di’an; E-mail: dani@sit.edu.cn
Abstract:
Different pulse feld strength and number of pulses were used for electroporation transformation of mature pollens of daylily (Hemerocallis sp.) ‘Black Eyed Stella’ to obtain an optimal parameter. The results showed that the optimal parameter of daylily pollen electroporation was 1 kV·cm–1 with 3 pulses
Key words: daylily (Hemerocallis sp.); pollen; electroporation transformation
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